Quantitative, radial diffusion slide assay for staphylocoagulase
نویسندگان
چکیده
منابع مشابه
Dye-diffusion test for semiquantitative staphylocoagulase assay.
Dye-diffusion test for semiquantitative staphylocoagulase assay. Ever since 1903, when Leob reported the ability of staphylococci to produce coagulase, an enzyme capable of clotting plasma, numerous workers have demonstrated the practical applicability of this phenomenon as an indicator of potential pathogenicity of these organisms. Two general procedures for coagulase determination are the tub...
متن کاملRadial diffusion assay of NAD- and NADP-dependent oxidoreductases.
With a radial diffusion assay we measured the activity of six NAD- and NADP-dependent oxidoreductases: alcohol, glucose-6-phosphate, hydroxysteroid, lactate and malate dehydrogenases, and glutathione reductase. The enzyme was allowed to diffuse for 24h in an agarose gel in which the substrate was incorporated, then reacted the pyridine nucleotide coenzyme. The size of an enzyme diffusion zone c...
متن کاملRapid and direct staphylocoagulase assay that uses a chromogenic substrate for identification of Staphylococcus aureus.
A rapid and direct assay of staphylocoagulase that uses chromogenic substrate Chromozym TH was developed for the routine identification of Staphylococcus aureus. False-positive and false-negative reactions were eliminated by measuring the activity of staphylocoagulase-prothrombin complex directly. The method was compared with three clotting assays. The results show that the new assay is specifi...
متن کاملA sensitive, single-radial diffusion autoradiographic zone size enhancement technique for the assay of influenza haemagglutinin.
A novel radial-diffusion technique in agarose gels is described which is applicable to the assay of small concentrations of influenza haemagglutinin (HA) antigen. The method is based on single-radial diffusion zone size enhancement (ZE) using autoradiography to demonstrate antigen--antibody reactions. The ZE technique is capable of reliably assaying concentrations of haemagglutinin as low as 0....
متن کاملApplication of the single radial diffusion test for assay of antibody to influenza type A viruses.
Single radial diffusion (SRD) tests for antibodies to influenza type A hemagglutinin, neuraminidase, nucleoprotein, and matrix protein antigens were compared with conventional hemagglutination inhibition, neuraminidase inhibition, and complement fixation tests. Sera used in this study were obtained in 1968-1969 from volunteers before and after vaccination and before and after an ensuing epidemi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1980
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.39.2.339-341.1980